![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4384003
Designed by: Zike Yao Group: iGEM22_HK_SSC (2022-10-09)
nitR_WT
This protein is expected to bind to valerolactam. It is the original cyclase. We have chosen to use the protein as it was reported that nitR_WT is used for the highly specific detection of lactam compounds against lactam biosynthetic intermediates. This protein is found present in Alcaligenes faecalis. [1]
Reference: [1] Yeom, S.-J.; Kim, M.; Kwon, K. K.; Fu, Y.; Rha, E.; Park, S.-H.; Lee, H.; Kim, H.; Lee, D.-H.; Kim, D.-M.; Lee, S.-G. A Synthetic Microbial Biosensor for High-Throughput Screening of Lactam Biocatalysts. Nature Communications 2018, 9 (1). https://doi.org/10.1038/s41467-018-07488-0.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |