Coding

Part:BBa_K4384003

Designed by: Zike Yao   Group: iGEM22_HK_SSC   (2022-10-09)


nitR_WT

This protein is expected to bind to valerolactam. It is the original cyclase. We have chosen to use the protein as it was reported that nitR_WT is used for the highly specific detection of lactam compounds against lactam biosynthetic intermediates. This protein is found present in Alcaligenes faecalis. [1]

Reference: [1] Yeom, S.-J.; Kim, M.; Kwon, K. K.; Fu, Y.; Rha, E.; Park, S.-H.; Lee, H.; Kim, H.; Lee, D.-H.; Kim, D.-M.; Lee, S.-G. A Synthetic Microbial Biosensor for High-Throughput Screening of Lactam Biocatalysts. Nature Communications 2018, 9 (1). https://doi.org/10.1038/s41467-018-07488-0.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None